学術雑誌論文 Mechanical properties of DNA origami nanoassemblies are determined by Holliday junction mechanophores

Shrestha, Prakash  ,  Emura, Tomoko  ,  Koirala, Deepak  ,  Cui, Yunxi  ,  Hidaka, Kumi  ,  Maximuck, William J  ,  Endo, Masayuki  ,  Sugiyama, Hiroshi  ,  Mao, Hanbin

44 ( 14 )  , pp.6574 - 6582 , 2016-08-19 , Oxford University Press
ISSN:1362-4962
内容記述
DNA nanoassemblies have demonstrated wide applications in various fields including nanomaterials, drug delivery and biosensing. In DNA origami, single-stranded DNA template is shaped into desired nanostructure by DNA staples that form Holliday junctions with the template. Limited by current methodologies, however, mechanical properties of DNA origami structures have not been adequately characterized, which hinders further applications of these materials. Using laser tweezers, here, we have described two mechanical properties of DNA nanoassemblies represented by DNA nanotubes, DNA nanopyramids and DNA nanotiles. First, mechanical stability of DNA origami structures is determined by the effective density of Holliday junctions along a particular stress direction. Second, mechanical isomerization observed between two conformations of DNA nanotubes at 10–35 pN has been ascribed to the collective actions of individual Holliday junctions, which are only possible in DNA origami with rotational symmetric arrangements of Holliday junctions, such as those in DNA nanotubes. Our results indicate that Holliday junctions control mechanical behaviors of DNA nanoassemblies. Therefore, they can be considered as ‘mechanophores’ that sustain mechanical properties of origami nanoassemblies. The mechanical properties observed here provide insights for designing better DNA nanostructures. In addition, the unprecedented mechanical isomerization process brings new strategies for the development of nano-sensors and actuators.
本文を読む

http://repository.kulib.kyoto-u.ac.jp/dspace/bitstream/2433/216360/1/nar_gkw610.pdf

このアイテムのアクセス数:  回

その他の情報