Journal Article An RNA-dependent RNA polymerase gene in bat genomes derived from an ancient negative-strand RNA virus.

Horie, Masayuki  ,  Kobayashi, Yuki  ,  Honda, Tomoyuki  ,  Fujino, Kan  ,  Akasaka, Takumi  ,  Kohl, Claudia  ,  Wibbelt, Gudrun  ,  Mühldorfer, Kristin  ,  Kurth, Andreas  ,  Müller, Marcel A.  ,  Corman, Victor M.  ,  Gillich, Nadine  ,  Suzuki, Yoshiyuki  ,  Schwemmle, Martin  ,  Tomonaga, Keizo

62016-05-13 , Springer Nature
Endogenous bornavirus-like L (EBLL) elements are inheritable sequences derived from ancient bornavirus L genes that encode a viral RNA-dependent RNA polymerase (RdRp) in many eukaryotic genomes. Here, we demonstrate that bats of the genus Eptesicus have preserved for more than 11. 8 million years an EBLL element named eEBLL-1, which has an intact open reading frame of 1, 718 codons. The eEBLL-1 coding sequence revealed that functional motifs essential for mononegaviral RdRp activity are well conserved in the EBLL-1 genes. Genetic analyses showed that natural selection operated on eEBLL-1 during the evolution of Eptesicus. Notably, we detected efficient transcription of eEBLL-1 in tissues from Eptesicus bats. To the best of our knowledge, this study is the first report showing that the eukaryotic genome has gained a riboviral polymerase gene from an ancient virus that has the potential to encode a functional RdRp.

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