学術雑誌論文 Formation of functional CENP-B boxes at diverse locations in repeat units of centromeric DNA in New World monkeys

Kugou, Kazuto  ,  Hirai, Hirohisa  ,  Masumoto, Hiroshi  ,  Koga, Akihiko

62016-06-13 , Nature Publishing Group
ISSN:2045-2322
内容記述
Centromere protein B, which is involved in centromere formation, binds to centromeric repetitive DNA by recognizing a nucleotide motif called the CENP-B box. Humans have large numbers of CENP-B boxes in the centromeric repetitive DNA of their autosomes and X chromosome. The current understanding is that these CENP-B boxes are located at identical positions in the repeat units of centromeric DNA. Great apes also have CENP-B boxes in locations that are identical to humans. The purpose of the present study was to examine the location of CENP-B box in New World monkeys. We recently identified CENP-B box in one species of New World monkeys (marmosets). In this study, we found functional CENP-B boxes in CENP-A-assembled repeat units of centromeric DNA in 2 additional New World monkeys (squirrel monkeys and tamarins) by immunostaining and ChIP-qPCR analyses. The locations of the 3 CENP-B boxes in the repeat units differed from one another. The repeat unit size of centromeric DNA of New World monkeys (340-350 bp) is approximately twice that of humans and great apes (171 bp). This might be, associated with higher-order repeat structures of centromeric DNA, a factor for the observed variation in the CENP-B box location in New World monkeys.
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http://repository.kulib.kyoto-u.ac.jp/dspace/bitstream/2433/215884/1/srep27833.pdf

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