Journal Article Oil-free hyaluronic acid matrix for serial femtosecond crystallography.

Sugahara, Michihiro  ,  Song, Changyong  ,  Suzuki, Mamoru  ,  Masuda, Tetsuya  ,  Inoue, Shigeyuki  ,  Nakane, Takanori  ,  Yumoto, Fumiaki  ,  Nango, Eriko  ,  Tanaka, Rie  ,  Tono, Kensuke  ,  Joti, Yasumasa  ,  Kameshima, Takashi  ,  Hatsui, Takaki  ,  Yabashi, Makina  ,  Nureki, Osamu  ,  Numata, Keiji  ,  Iwata, So

62016-04-18 , Nature Publishing Group
ISSN:2045-2322
Description
結晶を損傷しない新しいタンパク質結晶の輸送媒体を発見-タンパク質の結晶構造解析で新薬創生に一歩近づく. 京都大学プレスリリース. 2016-04-22.
The grease matrix was originally introduced as a microcrystal-carrier for serial femtosecond crystallography and has been expanded to applications for various types of proteins, including membrane proteins. However, the grease-based matrix has limited application for oil-sensitive proteins. Here we introduce a grease-free, water-based hyaluronic acid matrix. Applications for proteinase K and lysozyme proteins were able to produce electron density maps at 2.3-Å resolution.
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http://repository.kulib.kyoto-u.ac.jp/dspace/bitstream/2433/210352/1/srep24484.pdf

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