Journal Article Fluid shear triggers microvilli formation via mechanosensitive activation of TRPV6

Miura, Shigenori  ,  Sato, Koji  ,  Kato-Negishi, Midori  ,  Teshima, Tetsuhiko  ,  Takeuchi, Shoji

62015-11-13 , Nature Publishing Group
ISSN:2041-1723
Description
Microvilli are cellular membrane protrusions present on differentiated epithelial cells, which can sense and interact with the surrounding fluid environment. Biochemical and genetic approaches have identified a set of factors involved in microvilli formation; however, the underlying extrinsic regulatory mechanism of microvilli formation remains largely unknown. Here we demonstrate that fluid shear stress (FSS), an external mechanical cue, serves as a trigger for microvilli formation in human placental trophoblastic cells. We further reveal that the transient receptor potential, vanilloid family type-6 (TRPV6) calcium ion channel plays a critical role in flow-induced Ca2+ influx and microvilli formation. TRPV6 regulates phosphorylation of Ezrin via a Ca2+-dependent phosphorylation of Akt; this molecular event is necessary for microvillar localization of Ezrin in response to FSS. Our findings provide molecular insight into the microvilli-mediated mechanoresponsive cellular functions, such as epithelial absorption, signal perception and mechanotransduction.
Full-Text

http://repository.kulib.kyoto-u.ac.jp/dspace/bitstream/2433/210231/1/ncomms9871.pdf

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