学術雑誌論文 SsODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes

Yoshimi, Kazuto  ,  Kunihiro, Yayoi  ,  Kaneko, Takehito  ,  Nagahora, Hitoshi  ,  Voigt, Birger  ,  Mashimo, Tomoji

72016-01-20 , Nature Publishing Group
ISSN:2041-1723
内容記述
The CRISPR-Cas system is a powerful tool for generating genetically modified animals; however, targeted knock-in (KI) via homologous recombination remains difficult in zygotes. Here we show efficient gene KI in rats by combining CRISPR-Cas with single-stranded oligodeoxynucleotides (ssODNs). First, a 1-kb ssODN co-injected with guide RNA (gRNA) and Cas9 messenger RNA produce GFP-KI at the rat Thy1 locus. Then, two gRNAs with two 80-bp ssODNs direct efficient integration of a 5.5-kb CAG-GFP vector into the Rosa26 locus via ssODN-mediated end joining. This protocol also achieves KI of a 200-kb BAC containing the human SIRPA locus, concomitantly knocking out the rat Sirpa gene. Finally, three gRNAs and two ssODNs replace 58-kb of the rat Cyp2d cluster with a 6.2-kb human CYP2D6 gene. These ssODN-mediated KI protocols can be applied to any target site with any donor vector without the need to construct homology arms, thus simplifying genome engineering in living organisms.
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http://repository.kulib.kyoto-u.ac.jp/dspace/bitstream/2433/210211/1/ncomms10431.pdf

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