学術雑誌論文 Synergistic effect of ATP for RuvA-RuvB-Holliday junction DNA complex formation.

Iwasa, Takuma  ,  Han, Yong-Woon  ,  Hiramatsu, Ryo  ,  Yokota, Hiroaki  ,  Nakao, Kimiko  ,  Yokokawa, Ryuji  ,  Ono, Teruo  ,  Harada, Yoshie

52015-12-14 , Nature Publishing Group
ISSN:2045-2322
内容記述
The Escherichia coli RuvB hexameric ring motor proteins, together with RuvAs, promote branch migration of Holliday junction DNA. Zero mode waveguides (ZMWs) constitute of nanosized holes and enable the visualization of a single fluorescent molecule under micromolar order of the molecules, which is applicable to characterize the formation of RuvA-RuvB-Holliday junction DNA complex. In this study, we used ZMWs and counted the number of RuvBs binding to RuvA-Holliday junction DNA complex. Our data demonstrated that different nucleotide analogs increased the amount of Cy5-RuvBs binding to RuvA-Holliday junction DNA complex in the following order: no nucleotide, ADP, ATPγS, and mixture of ADP and ATPγS. These results suggest that not only ATP binding to RuvB but also ATP hydrolysis by RuvB facilitates a stable RuvA-RuvB-Holliday junction DNA complex formation.
本文を読む

http://repository.kulib.kyoto-u.ac.jp/dspace/bitstream/2433/207635/1/srep18177.pdf

このアイテムのアクセス数:  回

その他の情報