||Long-term maintenance of human induced pluripotent stem cells by automated cell culture system.
Konagaya, Shuhei ,
Ando, Takeshi ,
Yamauchi, Toshiaki ,
Suemori, HirofumiIwata, Hiroo
52015-11-17 , Nature Publishing Group
自動培養装置によるヒトiPS細胞の長期間培養に成功 . 京都大学プレスリリース. 2015-11-27.
Pluripotent stem cells, such as embryonic stem cells and induced pluripotent stem (iPS) cells, are regarded as new sources for cell replacement therapy. These cells can unlimitedly expand under undifferentiated conditions and be differentiated into multiple cell types. Automated culture systems enable the large-scale production of cells. In addition to reducing the time and effort of researchers, an automated culture system improves the reproducibility of cell cultures. In the present study, we newly designed a fully automated cell culture system for human iPS maintenance. Using an automated culture system, hiPS cells maintained their undifferentiated state for 60 days. Automatically prepared hiPS cells had a potency of differentiation into three germ layer cells including dopaminergic neurons and pancreatic cells.