||Thermosensitive Ion Channel Activation in Single Neuronal Cells by Using Surface-Engineered Plasmonic Nanoparticles.
Nakatsuji, Hirotaka ,
Numata, Tomohiro ,
Morone, Nobuhiro ,
Kaneko, Shuji ,
Mori, Yasuo ,
Imahori, HiroshiMurakami, Tatsuya
11729 , 2015-08-06 , Wiley
光を使って神経細胞の「痛み」感知を制御する手法を開発 -新しい鎮痛療法の可能性-. 京都大学プレスリリース. 2015-08-14.
Controlling cell functions using external photoresponsive nanomaterials has enormous potential for the development of cell-engineering technologies and intractable disease therapies, but the former currently requires genetic modification of the target cells. We present a method using plasma-membrane-targeted gold nanorods (pm-AuNRs) prepared with a cationic protein/lipid complex to activate a thermosensitive cation channel, TRPV1, in intact neuronal cells. Highly localized photothermal heat generation mediated by the pm-AuNRs induced Ca(2+) influx solely by TRPV1 activation. In contrast, the use of previously reported cationic AuNRs that are coated with a conventional synthetic polymer also led to photoinduced Ca(2+) influx, but this influx resulted from membrane damage. Our method provides an optogenetic platform without the need for prior genetic engineering of the target cells and might be useful for novel TRPV1-targeted phototherapeutic approaches.