Journal Article Regnase-1 and Roquin Regulate a Common Element in Inflammatory mRNAs by Spatiotemporally Distinct Mechanisms.

Mino, Takashi  ,  Murakawa, Yasuhiro  ,  Fukao, Akira  ,  Vandenbon, Alexis  ,  Wessels, Hans-Hermann  ,  Ori, Daisuke  ,  Uehata, Takuya  ,  Tartey, Sarang  ,  Akira, Shizuo  ,  Suzuki, Yutaka  ,  Vinuesa, Carola G  ,  Ohler, Uwe  ,  Standley, Daron M  ,  Landthaler, Markus  ,  Fujiwara, Toshinobu  ,  Takeuchi, Osamu

161 ( 5 )  , pp.1058 - 1073 , 2015-05-21 , Elsevier Inc.
ISSN:0092-8674
NCID:AA00600003
Description
炎症がRNA分解により制御されるメカニズムを解明 -二つのブレーキが炎症を巧妙にストップする-. 京都大学プレスリリース. 2015-05-22.
Regnase-1 and Roquin are RNA binding proteins essential for degradation of inflammation-related mRNAs and maintenance of immune homeostasis. However, their mechanistic relationship has yet to be clarified. Here, we show that, although Regnase-1 and Roquin regulate an overlapping set of mRNAs via a common stem-loop structure, they function in distinct subcellular locations: ribosome/endoplasmic reticulum and processing-body/stress granules, respectively. Moreover, Regnase-1 specifically cleaves and degrades translationally active mRNAs and requires the helicase activity of UPF1, similar to the decay mechanisms of nonsense mRNAs. In contrast, Roquin controls translationally inactive mRNAs, independent of UPF1. Defects in both Regnase-1 and Roquin lead to large increases in their target mRNAs, although Regnase-1 tends to control the early phase of inflammation when mRNAs are more actively translated. Our findings reveal that differential regulation of mRNAs by Regnase-1 and Roquin depends on their translation status and enables elaborate control of inflammation.
Full-Text

http://repository.kulib.kyoto-u.ac.jp/dspace/bitstream/2433/197955/1/j.cell.2015.04.029.pdf

Number of accesses :  

Other information