Thesis or Dissertation The Kidney Transcriptome and Proteome Defined by Transcriptomics and Antibody-Based Profiling

Habuka, Masato  ,  羽深, 将人

2015-09-24 , 新潟大学
学位の種類: 博士(医学). 報告番号: 甲第4069号. 学位記番号: 新大院博(医)甲第658号. 学位授与年月日: 平成27年9月24日
PLoS ONE. 2014, 9(12), e116125.
To understand renal functions and disease, it is important to define the molecular constituents of the various compartments of the kidney. Here, we used comparative transcriptomic analysis of all major organs and tissues in the human body, in combination with kidney tissue micro array based immunohistochemistry, to generate a comprehensive description of the kidney-specific transcriptome and proteome. A special emphasis was placed on the identification of genes and proteins that were elevated in specific kidney subcompartments. Our analysis identified close to 400 genes that had elevated expression in the kidney, as compared to the other analysed tissues, and these were further subdivided, depending on expression levels, into tissue enriched, group enriched or tissue enhanced. Immunohistochemistry allowed us to identify proteins with distinct localisation to the glomeruli (n=11), proximal tubules (n=120), distal tubules (n=9) or collecting ducts (n=8). Among the identified kidney elevated transcripts, we found several proteins not previously characterised or identified as elevated in kidney. This description of the kidney specific transcriptome and proteome provides a resource for basic and clinical research to facilitate studies to understand kidney biology and disease.

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