Thesis or Dissertation Study on Long-range Surface Plasmon Resonance Biosensor Using Electrospun Polymer Fibers

Netsuwan, Paphawadee

pp.1 - 85 , 2015-03-23 , 新潟大学
学位の種類: 博士(工学). 報告番号: 甲第4045号. 学位記番号: 新大院博(工)甲第427号. 学位授与年月日: 平成27年3月23日
The fabrication of long-range surface plasmon resonance (LR-SPR) immunosensor from water-stable electrospun polyacrylic acid (PAA) fibers was studied. LR-SPR setup consists of thin gold film and layer of Cytop which let it has larger evanescent field intensity and penetration depth. The water-stable electrospun PAA fibers produced by adding β-cyclodextrin as crosslinker followed by thermal treatment at 180 °C for 40 min were fabricated onto the LR-SPR sensor surface. The free carboxyl group of electrospun PAA fibers was activated to immobilize antibody-antigen in immunosensor application. The layer-by-layer (LbL) system on the electrospun PAA fibers was performed to further increase water-stability of fibers and number of active carboxyl group. Therefore, the LR-SPR immunosensor based on water-stable electrospun PAA fibers was used to detect of human immunoglobulin G (IgG).

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