||Differences in Healing Patterns of the Bone-Implant Interface between Immediately and Delayed-Placed Titanium Implants in Mouse Maxillae
Watanabe, Taisuke渡辺, 泰典
30 , 2015-03-23 , 新潟大学
学位の種類: 博士（歯学）. 報告番号: 甲第4017号. 学位記番号: 新大院博（歯）甲第330号. 学位授与年月日: 平成27年3月23日
Clinical Implant Dentistry and Related Research. Article first published online: 15 APR 2015
Background: There are no available data on the healing process at the bone-implant interface after an immediately placed implant. Purpose: This study was aimed to establish an animal experimental model of titanium implants placed in mouse maxillae and compare the healing pattern of the bone-implant interface after an immediately placed implant with that of a delayed placed implant. Materials and Methods: Maxillary first molars (M1) from 4-week-old mice were extracted and replaced with the implant following drilling (immediately placed group). In contrast, M1 from 2-week-old mice were extracted, followed by the drilling and implantation after 4 weeks (delayed placed group). The decalcified samples at 0-28 days after implantation were processed for immunohistochemistry, TUNEL assay, and TRAP histochemistry. The elements and bone volume of undecalcified samples were quantitatively analyzed by an Electron Probe Micro Analyzer. Results: Osseointegration was completed by 28 days after the procedure in both groups. There were no differences in the contact area, bone loss at the cervical area, and the rate of calcification at the bone-implant interface between the two groups. Conclusions: This study clarified no significant differences in the chronological healing process at the bone-implant interface between the two groups at the cellular level.