The possibilities for utility of oral cancer stem cells as new diagnostic and therapeutic targets
コウコウガン カンサイボウ オ ヒョウテキ トシタ シンキ シンダン チリョウホウ ノ カノウセイ
60 , 鹿児島大学
"The accumulating evidences that cancer stem cells (CSCs) involve in cancer invasion and metastasis gives us the concept of CSC targeted therapy. We have reported the involvement of epithelial–mesenchymal transition (EMT) and CSCs in invasion and metastasis model in vivo and in vitro with established adenoid cystic carcinoma (AdCC) cell lines. In this model, highly metastatic AdCC cell line shows not only the character of EMT, but also sphere forming ability and high expression of EMT related genes, stem cell markers and differentiation markers suggesting cancer stem cell (CSC) involved in EMT of AdCC. Surprisingly, silencing of T-box transcription factor Brachyury by shRNA results in down regulation of all EMT and stem cell markers. In addition, sphere forming ability, EMT character and tumorigenesis in vivo are simultaneously lost. We have also reported that Brachyury is a reliable prognostic factor for patients with oral carcinoma. We conclude that EMT directly link to CSC, and Brachyury is a one of the central regulator of EMT and CSC. These results suggest the possibility that Brachyury could be a possible diagnostic and therapeutic target for anti-CSC therapy for oral carcinoma in future."