Involvement of nervous system on the osteoblastic differentiation and function
シンケイケイ ノ コツガ サイボウ ブンカ・ キノウ エノ カンヨ ニツイテ
52 , 鹿児島大学
Recently the relationships between nervous system and bone metabolism have been revealed. Though recent evidences suggest that both sympathetic and sensory nerves affect bone metabolism, little is known about the involvement of neuropeptides on the differentiation of stem cells into osteoblastic (OB) cells. The aim of our study was to investigate the expression patterns of neuropeptide receptors at each differentiation stage to evaluate the putative effects of neuropeptides during the differentiation from mouse induced pluripotent stem (iPS) cells, embryoid bodies (EBs), to calcified tissueforming OB cells. Among neuropeptide receptors, β2-adrenergic receptor (AR) and calcitonin gene-related peptide receptor (CGRP-R) were expressed at all stages of cell differentiation, including the iPS cell stage, with peak expression occurring at the early osteoblastic differentiation stage. Another sensory nervous system receptor, neurokinin 1-R (NK1-R), was expressed mainly in the late osteoblastic differentiation stage. Furthermore, the expression of CGRP-R mRNA showed an additional small peak corresponding to EBs cultured for 3 days, suggesting that EBs may be affected by serum CGRP. These evidences suggest that the sympathetic nervous system receptor β2-AR and the sensory nervous system receptor CGRP-R may be involved in the differentiation of iPS cells into the osteoblastic lineage. NK1-R is likely to involve the late stage of osteoblastic cell differentiation. In other words each neuropeptide has an optimal period for the influence during the differentiation process of osteoblastic cells.