学術雑誌論文 Effects of elevated carbon dioxide on contraction force and proteome composition of sea urchin tube feet

Nasuchon, Nopparat  ,  Hirasaka, Katsuya  ,  Yamaguchi, Kenichi  ,  Okada, Jiro  ,  Ishimatsu, Atsushi

内容記述
This study examined how contraction force and protein profiles of the tube feet of the sea urchin (Pseudocentrotus depressus) were affected when acclimated to 400 (control), 2000 and 10,000 μatm CO2 for 48 days. Acclimation to higher CO2 conditions significantly reduced contraction force of the tube feet. Two-dimensional gel electrophoresis showed that eight spots changed in protein volume: six up-regulated and two down-regulated. Using matrix-assisted laser desorption/ionization-quadrupole ion trap-time of flight mass spectrometry, three up-regulated spots (tubulin beta chain, tropomyosin fragment, and actin N-terminal fragment) and two down-regulated spots (actin C-terminal fragment and myosin light chain) were identified. One possible interpretation of the results is that elevated CO2 weakened contraction of the tube feet muscle through an alteration of proteome composition, mainly associated with post-translational processing/proteolysis of muscle-related proteins.
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http://naosite.lb.nagasaki-u.ac.jp/dspace/bitstream/10069/37343/1/CBPd21_10.pdf

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