Journal Article Protective effect of porphyran isolated from discolored nori (Porphyra yezoensis) on lipopolysaccharide-induced endotoxin shock in mice

Nishiguchi, Tomoki  ,  Cho, Kichul  ,  Isaka, Shogo  ,  Ueno, Mikinori  ,  Jin, Jun-O  ,  Yamaguchi, Kenichi  ,  Kim, Daekyung  ,  Oda, Tatsuya

93 ( Part A )  , pp.1273 - 1278 , 2016-12 , Elsevier B.V.
Porphyran, a sulfated polysaccharide, isolated from discolored nori (Porphyra yezoensis) (dc-porphyran) and one fraction (F1) purified from dc-porphyran by DEAE-chromatography showed the protective effects on LPS-induced endotoxin shock in mice. Intraperitoneal (i.p.) treatment with dc-porphyran or F1 (100 mg/kg) 60 min prior to i.p. injection of LPS (30 mg/kg) completely protected mice from LPS lethality. At 10 mg/kg concentration, F1 demonstrated more protection than dc-porphyran. Intravenous (i.v.) challenge of LPS, even at 20 mg/kg, was more lethal than i.p. administration; i.v. injection of F1 (100 mg/kg) with LPS significantly improved the survival rate. However, i.v. dc-porphyran (100 mg/kg) produced an even lower survival rate than that of LPS alone. We examined pro-inflammatory mediators such as NO and TNF-α in serum. F1 significantly reduced the levels of these markers. Additionally, F1 significantly decreased the malondialdehyde level in the liver, a marker of oxidative stress, while dc-porphyran had almost no effect. Furthermore, F1 significantly decreased the production of TNF-α and NO in peritoneal exudate cells harvested from LPS-challenged mice, while dc-porphyran treatment showed a lesser decrease. Our results suggest that porphyran isolated from discolored nori, especially F1, is capable of suppressing LPS-induced endotoxin shock in vivo.

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