Potentiation of osteoclastgenesis by adipogenic conversion of bone marrow-derived mesenchymal stem cellsPotentiation of osteoclastgenesis by adipogenic conversion of bone marrow-derived mesenchymal stem cells 骨髄由来間葉系幹細胞の脂肪分化過程における破骨細胞分化誘導メカニズム
Bone marrow-derived mesenchymal stem cells (BMSCs) are the indispensable component of thebone marrow, being the common precursors for adipocytes and osteoblasts. We show here that adipogenicdifferentiation resulted in increase in the production of adipocyte markers, such as adiponectin,fatty-acid binding proteins (FABP4), peroxisome proliferator-activated receptor γ (PPARγ),as well as the receptor activator of nuclear-κB ligand (RANKL). Co-culture of osteoclast precursors(OCPs) with BMSCs-derived adipocytes significantly enhanced osteoclast differentiation withlow-dose RANKL, whose levels alone could not promote osteoclastogenesis. These results demonstratefor the first time that adipogenic differentiation of BMSCs plays a pivotal role in maintainingbone homeostasis.