Journal Article Enhanced catalytic activity of enzymes interacting with nanometric titanate nanosheets

Kamada, Kai  ,  Yamada, Akane  ,  Soh, Nobuaki

5 ( 104 )  , pp.85511 - 85516 , 2015-10-05 , Royal Society of Chemistry
The effect of the coexistence of titanate nanosheets (TNS) with nanometric lateral dimensions (ca. 3 nm), which were prepared through a hydrolysis reaction of titanium tetraisopropoxide, on the catalytic activity of horseradish peroxidase (HRP) was investigated as a function of solution pH. Especially in diluted HRP solutions with a pH range of 7-8, enzymatic reaction rate, i.e. maximum velocity (Vmax), in the conventional Michaelis-Menten equation, was significantly enhanced more than 2 times in the presence of TNS. In contrast, the increase in Vmax was not very large in acidic (pH = 4.0) and basic solutions (pH = 9.0). It was demonstrated that the TNS brought about peptization of aggregates composed of several HRP molecules in a diluted solution, causing an increase in the apparent HRP concentration participating in the enzymatic reaction. Moreover, the TNS activated superoxide dismutase (SOD) with O2 - scavenging performance.

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