We conducted an observational gait assessment and examined the relationship of gait deviations with physical function and walking ability. A total of 57 stroke patients admitted to a rehabilitation unit, who could walk unaided or under close supervision, were enrolled in the study (37 men; 20 women; 62.2±11.2 years of age; elapsed time since the onset of stroke: 90.9±39.9 days). We combined and partially modified several assessment forms used for gait evaluation. With regard to physical functions, we assessed: lower limb paralysis, sensory impairment, lower limb spasticity, range of motion, lower limb muscle strength, lower limb weight-bearing, and standing balance. We conducted a 10-meter walk test to assess patients’ walking ability. In order to identify variables that affect the total gait assessment score, a multiple regression analysis was performed. The following parameters were statistically associated with gait deviations: 10 meter walk time (standard partial regression coefficient=0.68), affected side hip abductor strength (–0.23), ankle plantar flexor spasticity (0.18), and maximum weight-bearing rate on the unaffected side (0.16). We established the relationship of gait deviations with physical function and walking ability.