Journal Article The Cardiorespiratory Responses to Inhalation and Pentobarbital Anesthesia in the Mouse

Chong, Han  ,  Ogata, Yoshiki  ,  Niwa, Hidetoshi  ,  Kushikata, Testuya  ,  Watanabe, Hiroyuki  ,  Imaizumi, Tadaatsu  ,  Hirota, Kazuyoshi  ,  Ono, Kyouichi  ,  Ohba, Takayoshi  ,  Murakami, Manabu

67 ( 1 )  , pp.77 - 85 , 2016-07-29 , 弘前大学大学院医学研究科・弘前医学会
Transgenic mice experiments have become increasingly popular to research human inherited disease.However, a number of Japanese researchers have difficulty with the selection of anesthesia, after the classificationof ketamine, probably the most used anesthesia, as a narcotic drug in 2006. Therefore, we compared the effects ofinhalation anesthesia (2% of isoflurane, sevoflurane and enflurane) and intraperitoneal pentobarbital anesthesia (50mg/kg) on the electrocardiogram( ECG) and blood oxygen saturation( SPO2) of mice. With inhalation anesthesia, theheart rate( HR) and SPO2 were within an acceptable range. In contrast, the HR significantly decreased after initiationof pentobarbital anesthesia, and gradually returned to a low rate. Importantly, pentobarbital anesthesia significantlylowered SPO2, and heart rate variability analysis showed unstable beat-to-beat intervals during pentobarbitalanesthesia, suggesting that inhalation anesthesia is more suitable for evaluation of cardiorespiratory responsesthan pentobarbital anesthesia. During anesthesia, propranolol, a ????-adrenergic blocker, significantly decreased heartrate. Atropine, a parasympathetic blocker, also significantly increased heart rate. Our data suggest that inhalation  anesthesia is suitable for cardiorespiratory analysis in mice.

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