Journal Article Capillary zone electrophoresis determination of fluoride in seawater using transient isotachophoresis

Fukushi, Keiichi  ,  Fujita, Yuki  ,  Nonogaki, Junpei  ,  Tsujimoto, Jun-ichi  ,  Hattori, Takanari  ,  Inui, Hideyuki  ,  Beskoski, Vladimir P.  ,  Hotta, Hiroki  ,  Hayashi, Mitsuru  ,  Nakano, Takeshi

410 ( 6 )  , pp.1825 - 1831 , 2018-02 , Springer Heidelberg
We developed capillary zone electrophoresis (CZE) with indirect UV detection for the determination of fluoride (F−) in seawater using transient isotachophoresis (tITP) as an on-line concentration procedure. A method of correcting sample salinity effects was also proposed so that F− concentrations were obtained using a calibration graph. The proposed method is simple: it requires no sample pretreatment aside from dilution. The following optimum conditions were established: background electrolyte (BGE), 5 mM 2,6-pyridinedicarboxylic acid (PDC) adjusted to pH 3.5 containing 0.03% m/v hydroxypropyl methylcellulose (HPMC); detection wavelength, 200 nm; vacuum (50 kPa) injection period of sample, 5 s (254 nL); and applied voltage, 23 kV with the sample inlet side as the cathode. The limit of detection (LOD, S/N = 3) and limit of quantification (LOQ, S/N = 10) for F− reached 0.024 and 0.070 mg/L, respectively. The respective values of the relative standard deviation (RSD) of the peak area, peak height, and migration time for F− were 2.5, 3.4, and 0.30%. The proposed method was applied for the determination of F− in seawater samples collected from coastal waters of western Japan during August 26–28, 2014. Both results obtained using standard addition method and a calibration graph agreed with those obtained using a conventional spectrophotometric method.

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