学術雑誌論文 Involvement of WNT Signaling in the Regulation of Gestational Age-Dependent Umbilical Cord-Derived Mesenchymal Stem Cell Proliferation

Iwatani, Sota  ,  Shono, Akemi  ,  Yoshida, Makiko  ,  Yamana, Keiji  ,  Thwin, Khin Kyae Mon  ,  Kuroda, Jumpei  ,  Kurokawa, Daisuke  ,  Koda, Tsubasa  ,  Nishida, Kosuke  ,  Ikuta, Toshihiko  ,  Fujioka, Kazumichi  ,  Mizobuchi, Masami  ,  Taniguchi-Ikeda, Mariko  ,  Morioka, Ichiro  ,  Iijima, Kazumoto  ,  Nishimura, Noriyuki

2017p.8749751 , 2017-09-12 , Hindawi Publishing Corporation
ISSN:1687966X16879678
内容記述
Mesenchymal stem cells (MSCs) are a heterogeneous cell population that is isolated initially from the bone marrow (BM) and subsequently almost all tissues including umbilical cord (UC). UC-derived MSCs (UC-MSCs) have attracted an increasing attention as a source for cell therapy against various degenerative diseases due to their vigorous proliferation and differentiation. Although the cell proliferation and differentiation of BM-derived MSCs is known to decline with age, the functional difference between preterm and term UC-MSCs is poorly characterized. In the present study, we isolated UC-MSCs from 23 infants delivered at 22-40 weeks of gestation and analyzed their gene expression and cell proliferation. Microarray analysis revealed that global gene expression in preterm UC-MSCs was distinct from term UC-MSCs. WNT signaling impacts on a variety of tissue stem cell proliferation and differentiation, and its pathway genes were enriched in differentially expressed genes between preterm and term UC-MSCs. Cell proliferation of preterm UC-MSCs was significantly enhanced compared to term UC-MSCs and counteracted by WNT signaling inhibitor XAV939. Furthermore, WNT2B expression in UC-MSCs showed a significant negative correlation with gestational age (GA). These results suggest that WNT signaling is involved in the regulation of GAdependent UC-MSC proliferation.
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http://www.lib.kobe-u.ac.jp/repository/90004405.pdf

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