学術雑誌論文 Nanosecond pump-probe device for time-resolved serial femtosecond crystallography developed at SACLA

Kubo, Minoru  ,  Nango, Eriko  ,  Tono, Kensuke  ,  Kimura, Tetsunari  ,  Owada, Shigeki  ,  Song, Changyong  ,  Mafune, Fumitaka  ,  Miyajima, Ken  ,  Takeda, Yoshihiro  ,  Kohno, Jun-ya  ,  Miyauchi, Naoya  ,  Nakane, Takanori  ,  Tanaka, Tomoyuki  ,  Nomura, Takashi  ,  Davidsson, Jan  ,  Tanaka, Rie  ,  Murata, Michio  ,  Kameshima, Takashi  ,  Hatsui, Takaki  ,  Joti, Yasumasa  ,  Neutze, Richard  ,  Yabashi, Makina  ,  Iwata, So

24 ( 5 )  , pp.1086 - 1091 , 2017-09-01 , International Union of Crystallography
ISSN:0909049516005775
内容記述
X-ray free-electron lasers (XFELs) have opened new opportunities for timeresolved X-ray crystallography. Here a nanosecond optical-pump XFEL-probe device developed for time-resolved serial femtosecond crystallography (TRSFX) studies of photo-induced reactions in proteins at the SPring-8 Angstrom Compact free-electron LAser (SACLA) is reported. The optical-fiber-based system is a good choice for a quick setup in a limited beam time and allows pump illumination from two directions to achieve high excitation efficiency of protein microcrystals. Two types of injectors are used: one for extruding highly viscous samples such as lipidic cubic phase (LCP) and the other for pulsed liquid droplets. Under standard sample flow conditions from the viscous-sample injector, delay times from nanoseconds to tens of milliseconds are accessible, typical time scales required to study large protein conformational changes. A first demonstration of a TR-SFX experiment on bacteriorhodopsin in bicelle using a setup with a droplet-type injector is also presented.
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http://www.lib.kobe-u.ac.jp/repository/90004340.pdf

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