||Capillary zone electrophoresis determination of aniline and pyridine in sewage samples using transient isotachophoresis with a system-induced terminator
Hattori, Takanari ,
Okamura, Hideo ,
Asaoka, SatoshiFukushi, Keiichi
Journal of Chromatography A
137 , 2017-08-18 , Elsevier
Transient isotachophoresis (tITP) with a system-induced terminator (SIT) was developed for capillary zone electrophoresis (CZE) determination of aniline (An(+)) and pyridine (Py+) in sewage samples. After sample injection, a water vial was set at the sample-inlet side. Then voltage was applied to generate a system-induced terminator (H+). Experiments and simulations revealed a concentration effect by tITP with an SIT: background electrolyte (BGE) -100 mM acetic acid (AcOH) and 50 mM NaOH (pH 4.6); detection wavelength -200 nm for An(+) and 254 nm for Py+; vacuum injection period -15 s (190 nL); SIT generation -10 kV applied for 80 s with the sample inlet side anode; separation voltage -20 kV with the sample inlet side anode. The limits of detection (LODs, S/N = 3) of An(+) and Py+ respectively reached 10 and 42 mu g/L, with good repeatability (peak area RSDs <= 6.9%) and calibration graph linearity (R-2 = 0.9997). The proposed method was applied for determination of An(+) and Py+ in sewage samples. Recoveries of An(+) (0.50 mg/L) and Py+ (2.0 mg/L) in spiked sewage samples were 94-104%.