学術雑誌論文 Impaired lymphocyte trafficking in mice deficient in the kinase activity of PKN1

Mashud, Rana  ,  Nomachi, Akira  ,  Hayakawa, Akihide  ,  Kubouchi, Koji  ,  Danno, Sally  ,  Hirata, Takako  ,  Matsuo, Kazuhiko  ,  Nakayama, Takashi  ,  Satoh, Ryosuke  ,  Sugiura, Reiko  ,  Abe, Manabu  ,  Sakimura, Kenji  ,  Wakana, Shigeharu  ,  Ohsaki, Hiroyuki  ,  Kamoshida, Shingo  ,  Mukai, Hideyuki

7p.7663 , 2017-08-09 , Nature Publishing Group
ISSN:2045232220452322
内容記述
Knock-in mice lacking PKN1 kinase activity were generated by introducing a T778A point mutation in the catalytic domain. PKN1[T778A] mutant mice developed to adulthood without apparent external abnormalities, but exhibited lower T and B lymphocyte counts in the peripheral blood than those of wild-type (WT) mice. T and B cell development proceeded in an apparently normal fashion in bone marrow and thymus of PKN1[T778A] mice, however, the number of T and B cell counts were significantly higher in the lymph nodes and spleen of mutant mice in those of WT mice. After transfusion into WT recipients, EGFP-labelled PKN1[T778A] donor lymphocytes were significantly less abundant in the peripheral circulation and more abundant in the spleen and lymph nodes of recipient mice compared with EGFP-labelled WT donor lymphocytes, likely reflecting lymphocyte sequestration in the spleen and lymph nodes in a cell-autonomous fashion. PKN1[T778A] lymphocytes showed significantly lower chemotaxis towards chemokines and sphingosine 1-phosphate (S1P) than WT cells in vitro. The biggest migration defect was observed in response to S1P, which is essential for lymphocyte egress from secondary lymphoid organs. These results reveal a novel role of PKN1 in lymphocyte migration and localization.
本文を読む

http://www.lib.kobe-u.ac.jp/repository/90004212.pdf

このアイテムのアクセス数:  回

その他の情報