Departmental Bulletin Paper Potency of an inactivated influenza vaccine prepared from A/duck/Mongolia/245/2015 (H10N3) against H10 influenza virus infection in a mouse model

Suzuki, Mizuho  ,  Okamatsu, Masatoshi  ,  Fujimoto, Yuri  ,  Hiono, Takahiro  ,  Matsuno, Keita  ,  Kida, Hiroshi  ,  Sakoda, Yoshihiro

66 ( 1 )  , pp.29 - 41 , 2018-02
The H10N8 influenza virus became a threat to public health when cases of fatal infections were identified in China in 2013 and 2014. Thus, genetic and antigenic characterization of H10 influenza viruses and development of an appropriate vaccine are essential to prepare for a future pandemic by H10 influenza viruses. However, current information regarding these properties of H10 influenza viruses circulating in birds is limited. In this study, genetic analysis of H10 influenza viruses revealed that the viruses recently circulating in wild birds in East Asia are genetically close to human H10N8 influenza viruses. Furthermore, the antigenicity of H10 influenza viruses was stable among the viruses circulating in birds. An inactivated vaccine was prepared from A/duck/Mongolia/245/2015 (H10N3), which is genetically and antigenically close to the human H10 influenza viruses. The vaccine induced sufficient neutralizing antibodies against homologous and heterologous viruses in mice. The inactivated vaccine induced protective immunity sufficient to reduce the impact of challenges with A/duck/Hokkaido/W87/2007 (H10N2), which is pathogenic strain in mice. This study demonstrates that the inactivated whole virus particle vaccine prepared from viruses isolated from wild birds would be useful against a future pandemic influenza by H10 influenza viruses.

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