Journal Article Characterization of a Novel Bat Adenovirus Isolated from Straw-Colored Fruit Bat (Eidolon helvum)

Ogawa, Hirohito  ,  Kajihara, Masahiro  ,  Nao, Naganori  ,  Shigeno, Asako  ,  Fujikura, Daisuke  ,  Hang'ombe, Bernard M.  ,  Mweene, Aaron S.  ,  Mutemwa, Alisheke  ,  Squarre, David  ,  Yamada, Masao  ,  Higashi, Hideaki  ,  Sawa, Hirofumi  ,  Takada, Ayato

9 ( 12 )  , p.371 , 2017-12 , MDPI
ISSN:1999-4915
Description
Bats are important reservoirs for emerging zoonotic viruses. For extensive surveys of potential pathogens in straw-colored fruit bats (Eidolon helvum) in Zambia, a total of 107 spleen samples of E. helvum in 2006 were inoculated onto Vero E6 cells. The cell culture inoculated with one of the samples (ZFB06-106) exhibited remarkable cytopathic changes. Based on the ultrastructural property in negative staining and cross-reactivity in immunofluorescence assays, the virus was suspected to be an adenovirus, and tentatively named E. helvum adenovirus 06-106 (EhAdV 06-106). Analysis of the full-length genome of 30,134 bp, determined by next-generation sequencing, showed the presence of 28 open reading frames. Phylogenetic analyses confirmed that EhAdV 06-106 represented a novel bat adenovirus species in the genus Mastadenovirus. The virus shared similar characteristics of low G + C contents with recently isolated members of species Bat mastadenoviruses E, F and G, from which EhAdV 06-106 diverged by more than 15% based on the distance matrix analysis of DNA polymerase amino acid sequences. According to the taxonomic criteria, we propose the tentative new species name “Bat mastadenovirus H”. Because EhAdV 06-106 exhibited a wide in vitro cell tropism, the virus might have a potential risk as an emerging virus through cross-species transmission.
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https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/68273/1/viruses-09-00371.pdf

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