Journal Article Periodontal tissue engineering by nano beta-tricalcium phosphate scaffold and fibroblast growth factor-2 in one-wall infrabony defects of dogs
Periodontal tissue engineering by nano beta-TCP scaffold and FGF-2 in 1-wall infrabony defects of dogs
Periodontal healing by nano β-TCP and FGF-2

Ogawa, Kosuke  ,  Miyaji, Hirofumi  ,  Kato, Akihito  ,  Kosen, Yuta  ,  Momose, Takehito  ,  Yoshida, Takashi  ,  Nishida, Erika  ,  Miyata, Saori  ,  Murakami, Shusuke  ,  Takita, Hiroko  ,  Fugetsu, Bunshi  ,  Sugaya, Tsutomu  ,  Kawanami, Masamitsu

51 ( 6 )  , pp.758 - 767 , 2016-12 , Wiley-Blackwell
Background and Objective: Nanoparticle bioceramics are being investigated for biomedical applications. We fabricated a regenerative scaffold comprising type I collagen and beta-tricalcium phosphate (β-TCP) nanoparticles. Fibroblast growth factor-2 (FGF-2) is a bioeffective signaling molecule that stimulates cell proliferation and wound healing. This study examined the effects on bioactivity from a nano-β-TCP/collagen scaffold loaded with FGF-2, particularly on periodontal tissue wound healing. Material and Methods: β-TCP was pulverized into nano-sized particles (84 nm) and was then dispersed. Nano-β-TCP scaffold was prepared by coating the surface of a collagen scaffold with a nano-sized β-TCP dispersion. Scaffolds were characterized using scanning electron microscopy (SEM), compressive testing, cell seeding, and rat subcutaneous implant testing. Then nano-β-TCP scaffold, nano-β-TCP scaffold loaded with FGF-2, and non-coated collagen scaffold were implanted into a dog 1-wall infrabony defect model. Histological observations were made at 10 d and 4 wk post-surgery. Results: Results of SEM observation show that TCP nanoparticles were attached to collagen fibers. Nano-β-TCP scaffold showed higher compressive strength and cytocompatibility than non-coated collagen scaffold. Rat subcutaneous implant tests showed that DNA contents of infiltrating cells in the nano-β-TCP scaffold and FGF2-loaded scaffold were each approximately 2.8-fold and 3.7-fold greater than the collagen scaffold. Histological samples from the periodontal defect model showed about five-fold greater periodontal tissue repair following implantation of the nano-β-TCP scaffold loaded with FGF-2 rather than the collagen scaffold. Conclusion: The β-TCP nanoparticle coating strongly improved the collagen scaffold bioactivity. Nano-β-TCP scaffolds with FGF-2 are anticipated for use in periodontal tissue engineering.

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