Journal Article Bone regeneration around N-acetyl cysteine-loaded nanotube titanium dental implants

Lee, Young-Hee  ,  Bhattarai, Govinda  ,  Park, Il-Song  ,  Kim, Ga-Ram  ,  Kim, Go-Eun  ,  Lee, Min-Ho  ,  Kim, In-Ju  ,  Seo, Jae-Min  ,  Ahn, Seung-Geun  ,  Yi, Ho-Keun

38 ( Special issue )  , pp.9 - 19 , 2017-09 , 北海道歯学会
New strategies involving drugs loading onto implant surfaces are required to enhance osseointegration and shorten healing time after implantation. In this study, we examined the feasibility of N -acetyl cysteine (NAC)-loaded nanotube titanium (NLN-Ti) implants as a potential drug delivery system. To determine the effect of NLN-Ti in in vitro and in vivo, viability and ROS formation was assessed and enzyme-linked immunosorbant assay (ELISA), Western blot, micro-computed tomography (μ-CT), hematoxylin and eoxin (H&E) staining and immunohistochemical (IHC) analysis were performed. In vitro, cell viability was increased and inflammatory responses and reduced oxidative stress-related defense were decreased with MC 3T3-E1 cells exposed to a sustained release of NAC from NLN-Ti implants. Following NLN-Ti implant installation, μ-CT and histomorphometric analysis revealed an increase of newly formed bone volume and bone mineral density in the mandibles of Sprague Dawley rats and beagle dogs. Relatively well-formed new bone was demonstrated in close contact to the NLN-Ti implant surface by H&E staining. IHC revealed a significantly higher expression of bone morphogenetic protein-2, -7 and heme oxygenase-1, and reduced the expression of the receptor activator of nuclear factor-kappa B ligand. The data indicate that NLN-Ti implants enhance osseointegration and highlight the value of the animal model in assessing diverse biological responses to dental implants.

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