Departmental Bulletin Paper The DNA methylation inhibitor 5-aza-2’-deoxycytidine retards cell growth and alters gene expression in canine mammary gland tumor cells

Ito, Tsuyoshi  ,  Kaneda, Masahiro

65 ( 3 )  , pp.159 - 165 , 2017-08 , Faculty of Veterinary Medicine, Hokkaido University
Disruption of gene expression by DNA methylation changes is widely involved in tumorigenesis. Here,to investigate DNA methylation changes in canine, we treated a canine mammary gland tumor cell line with a DNA methylation inhibitor, 5-aza-2’-deoxycytidine (5-aza). Cell growth was significantly retarded following 5-aza treatment and the epithelial marker genes CDH1 and KRT18 were significantly up-regulated, whereas the mesenchymal marker genes CDH2 and VIM were significantly downregulated. We also found a significant decrease in DNA methylation level in the CDH1 promoter region by 5-aza treatment. These results showed for the first time in canine mammary gland tumor cells that inhibition of DNA methylation caused cell growth retardation and affected epithelial mesenchymal transition-related gene expression via changes in DNA methylation level.

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