||Effects of semen preservation procedure in egg yolk-tris based extender on bull spermatozoa characteristics
Kanno, Chihiro ,
Yanagawa, Yojiro ,
Takahashi, Yoshiyuki ,
Katagiri, SeijiNagano, Masashi
Japanese Journal of Veterinary Research
157 , 2017-08 , Faculty of Veterinary Medicine, Hokkaido University
To verify the dynamics of damages to spermatozoa during semen freezing, characteristics of spermatozoa collected from 3 Japanese black bulls were evaluated by using fluorescent staining. Pre-diluted sample showed the highest proportion of spermatozoa with intact plasma membrane, intact acrosome and high mitochondrial potential. The proportion of spermatozoa with intact plasma membrane, intact acrosome, and low mitochondrial membrane potential were higher after cooling to 4°C than the other processes. During cooling preservation examined in this study, the proportion of spermatozoa with damaged acrosome increased. These results lead us to speculate that, during cooling process, spermatozoa may be firstly injured to mitochondrial membrane, and low mitochondrial function may cause the impairment of plasma membrane and subsequent cell death with acrosomal damage.