Departmental Bulletin Paper Molecular characterization of the lymphocyte activation gene-3 (LAG-3, CD223) of swamp- and riverine-type water buffaloes (Bubalus bubalis)

Rivera, Shanemae M.  ,  Padiernos, Ryan Bismark C.  ,  Abella, Evaristo A.  ,  Konnai, Satoru  ,  Mingala, Claro N.

65 ( 2 )  , pp.65 - 74 , 2017-05 , Faculty of Veterinary Medicine, Hokkaido University
The present study was conducted to characterize LAG-3 of swamp- and riverine-type water buffaloes by DNA sequencing, homology and phylogenetic analysis. Bubaline LAG-3 sequence contained an open reading frame of 1551 nucleotide, encoding a polypeptide of 516 amino acids. Nucleotide and amino acid sequence homology of LAG-3 revealed 76-96% and 61-94% identity in water buffalo to that of other mammals, respectively. LAG-3 protein sequence of water buffalo contained four extracellular domains, a transmembrane domain and different conserved regions. There were three N-glycosylation sites, two sequence motifs: ‘RGD’ and ‘WXC’ motif and five cysteine residues located at different positions of extracellular region. Likewise, the possible serine phosphorylation site and the ‘KTGELE’ inhibitory motif were found in the intracellular region of bubaline LAG-3. However, one highly conserved cysteine residue in mammalian LAG-3 was replaced by tyrosine in both swamp- and riverine-type water buffaloes. Phylogenetic analysis generated high bootstrap value between the two types of water buffalo which further confirmed the degree of relationship between bubaline species. This was the first report that describe the genetic characteristic of LAG-3 in swamp- and riverine-type water bufffaloes.

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