学術雑誌論文 Decreased RNA-binding motif 5 expression is associated with tumor progression in gastric cancer

Kobayashi, Takahiko  ,  Ishida, Junich  ,  Shimizu, Yuichi  ,  Kawakami, Hiroshi  ,  Suda, Goki  ,  Muranaka, Tetsuhito  ,  Komatsu, Yoshito  ,  Asaka, Masahiro  ,  Sakamoto, Naoya

39 ( 3 ) 2017-03 , SAGE Publications
ISSN:1010-4283
内容記述
RNA-binding motif 5 is a putative tumor suppressor gene that modulates cell cycle arrest and apoptosis. We recently demonstrated that RNA-binding motif 5 inhibits cell growth through the p53 pathway. This study evaluated the clinical significance of RNA-binding motif 5 expression in gastric cancer and the effects of altered RNA-binding motif 5 expression on cancer biology in gastric cancer cells. RNA-binding motif 5 protein expression was evaluated by immunohistochemistry using the surgical specimens of 106 patients with gastric cancer. We analyzed the relationships of RNA-binding motif 5 expression with clinicopathological parameters and patient prognosis. We further explored the effects of RNA-binding motif 5 downregulation with short hairpin RNA on cell growth and p53 signaling in MKN45 gastric cancer cells. Immunohistochemistry revealed that RNA-binding motif 5 expression was decreased in 29 of 106 (27.4%) gastric cancer specimens. Decreased RNA-binding motif 5 expression was correlated with histological differentiation, depth of tumor infiltration, nodal metastasis, tumor-node-metastasis stage, and prognosis. RNA-binding motif 5 silencing enhanced gastric cancer cell proliferation and decreased p53 transcriptional activity in reporter gene assays. Conversely, restoration of RNA-binding motif 5 expression suppressed cell growth and recovered p53 transactivation in RNA-binding motif 5-silenced cells. Furthermore, RNA-binding motif 5 silencing reduced the messenger RNA and protein expression of the p53 target gene p21. Our results suggest that RNA-binding motif 5 downregulation is involved in gastric cancer progression and that RNA-binding motif 5 behaves as a tumor suppressor gene in gastric cancer.
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https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/66569/3/Supplementary_Material_2.pdf

https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/66569/2/Supplementary_Material.pdf

https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/66569/1/1010428317694547.pdf

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