Journal Article Caspase-dependent cleavage regulates protein levels of Epstein-Barr virus-derived latent membrane protein 1

Togi, Sumihito  ,  Hatano, Yosuke  ,  Muromoto, Ryuta  ,  Kawanishi, Eri  ,  Ikeda, Osamu  ,  Hirashima, Koki  ,  Kon, Shigeyuki  ,  Kitai, Yuichi  ,  Yasui, Teruhito  ,  Oritani, Kenji  ,  Matsuda, Tadashi

590 ( 6 )  , pp.808 - 818 , 2016-03 , Wiley-Blackwell
ISSN:0014-5793
Description
Epstein-Barr virus (EBV)-encoded latent membrane protein-1 (LMP1) plays pathogenic roles in EBV-related diseases. Thus, host cells employ several mechanisms to regulate LMP1 functions, and we previously reported possible regulation by signal transducing adaptor protein-2 as well as BS69. Here, we found that caspase-3 mainly degraded LMP1 proteins in HeLa cells, leading to decreased NF-kappa B and STAT3 activation. Caspase-3 cleaved the consensus DNTD sequences in the CTAR3 region of LMP1. Of importance, LMP1 expression strongly enhanced caspase-3 activity. Taken together, the reduction of LMP1 protein levels by caspases is likely to be a newly identified host defense against EBV infection.
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http://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/64895/1/manuscript.pdf

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