||Identifying bioactive properties of graphene oxide scaffold for bone tissue engineering therapy
Graphene oxide (GO), consisting of a carbon monolayer, has been widely investigated for tissueengineering platforms because of its unique properties. For this study, we fabricated GO-appliedscaffold and assessed the physical properties and cellular and tissue behaviors in the scaffold. Inaddition, a preclinical test was conducted to ascertain whether the GO scaffold promoted boneinduction in dog tooth extraction sockets. Results showed that GO application improved the physicalstrength, enzyme resistance, and adsorption of calcium and proteins. In a cytocompatibility test, GOapplication was found to increase osteoblastic MC3T3-E1 cell proliferation significantly. In addition,an assessment of rat subcutaneous tissue response showed that implantation of GO scaffoldstimulated cellular in-growth behavior, suggesting that the GO scaffold exhibited goodbiocompatibility. Particularly, the infiltration of ED2- positive (M2) macrophages and blood vesselswere prominent in the GO scaffold. In a dog bone-formation test, the GO scaffold implantationenhanced bone formation. New bone formation following GO scaffold implantation was enhancedapproximately five-fold compared to that in control. These results suggest that GO providedbiocompatibility and bone forming capability for the scaffold. Therefore, the GO scaffold is expectedto be available for bone tissue engineering therapy.
Hokkaido University（北海道大学）. 博士(歯学)