Journal Article Limnochorda pilosa gen. nov., sp nov., a moderately thermophilic, facultatively anaerobic, pleomorphic bacterium and proposal of Limnochordaceae fam. nov., Limnochordales ord. nov and Limnochordia classis nov in the phylum Firmicutes

Watanabe, Miho  ,  Kojima, Hisaya  ,  Fukui, Manabu

A novel facultatively anaerobic bacterium, strain HC45(T), was isolated from sediment of a brackish meromictic lake in Japan, Lake Harutori. Cells were pleomorphic, and filamentous bodies were 5-100 mu m in length. For growth, the optimum pH was 7.0 and the optimum temperature was 45-50 degrees C. The G+C content of the genomic DNA was 71 mol%. iso-C-15 (:) (0) and anteiso-C-15 : (0) were the major components in the cellular fatty acid profile. The predominant respiratory quinone was MK-7. Strain HC45(T) shared very low 16S rRNA gene sequence similarity with cultivated strains (<= 85 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate was distantly related to members of the family Symbiobacteriaceae and family XVII Incertae Sedis in the class Clostridia, and they formed a cluster separate from canonical species of the phylum Firmicutes. These results indicated that strain HC45(T) should not be placed in any existing class of the phylum Firmicutes. On the basis of phylogenetic and phenotypic characterization, Limnochorda pilosa gen. nov., sp. nov. is proposed with HC45(T) (=NBRC 110152(T)=DSM 28787T) as the type strain, as the first representative of novel taxa, Limnochordales ord. nov., Limnochordaceae fam. nov. in Limnochordia classis. nov.

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