||Diversity of lactic acid bacteria isolated from raw milk in Elsharkia province, Egypt
Alnakip, Mohamed E. A. ,
Mohamed, Asmaa S. ,
Kamal, Rania M.Elbadry, Seham
Japanese Journal of Veterinary Research
S30 , 2016-04 , Graduate School of Veterinary Medicine, Hokkaido University
A total of 50 raw cow’s milk samples were collected from different areas of Elsharkia province, Egypt for characterizing lactic acid bacteria (LAB) load. Using 16S rRNA gene sequencing, a total of 41 LAB isolates have been identified corresponding to Enterococcus sp. (51.22 %) as the most predominant LAB genus, followed in order by Aerococcus (26.82 %), Lactococcus (7.32 %), Lactobacillus (7.32 %), Leuconostic (4.88 %) and Pediococcus (2.44 %) genera. All isolates were identified to species level with exception of one strain (Lc. lactis subsp. cremoris) that has been assigned to subspecies. The phylogenetic dendrogram created has allowed good discrimination between all isolated LAB species identified with this study. Results showed a wide diversity among isolated LAB from raw milk in Elsharkia province. The impact of LAB presence in raw cow’s milk on dairy safety has been discussed.