Departmental Bulletin Paper Genotypic detection of the fimA gene and virulence associated genes of salmonellea in cattle

EL-Sherbiny, Fatma  ,  El-Bialy, Amany I.  ,  Salwa, M. H.  ,  Ammar, A. M.

64 ( 2 )  , pp.S45 - S51 , 2016-04 , Graduate School of Veterinary Medicine, Hokkaido University
ISSN:0047-1917
NCID:AA0069128X
Description
Bacteriological examination of 640 fecal samples collected from apparent healthy (250) and diarrheic cattle (390) for the presence of Salmonella organism revealed that 12(1.87%) Salmonella isolates were isolated. The highest percentage rate for Salmonella recovery was in diarrheic cow calves (3.6%) followed by apparent healthy cow (2.2%). Serological identification of Salmonella isolates revealed that the most prevalent serovers were S.Typhimrium(2), S.Belgdam(2) and S.Lomita(2). Followed by S.Larochelle, S.Virchow, S.Infantis, S.Derpy, S.Anatum and S.Berzany one isolate for each. The goal of this study was to evaluate the suitability of the fimA gene amplification by Real-time PCR as a specific method for detection of salmonellae from fecal samples. It was noticed that 3 fecal samples were positive by Rt-PCR more than cultural method. On the other hand 2 samples were positive by cultural method and could not be detected by fimA Rt-PCR. pef and avrA virulence genes were detected in 7 and 4 out of 12 culture positive samples. In conclusion, Rt PCR is rapid, reproducible, and robust method for detection of genus Salmonella. However it should be taken in consideration that 10-20% of isolates could be missed due to absence of fimA gene. This may be corrected by application of multiplex Rt-PCR using more virulence genes.
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