Departmental Bulletin Paper Detection of SNPs in growth hormone and insulin like growth factor -1 genes in two divergently selected lines of Japanese quail

El-Bayomi, Khairy M.  ,  El-Tarabany, Mahmoud S.  ,  Asr, Mohammed A. F.  ,  Awad, Ashraf  ,  Roushdy, El-Shimaa M.

64 ( 2 )  , pp.S53 - S57 , 2016-04 , Graduate School of Veterinary Medicine, Hokkaido University
The Japanese quail (Coturnix japonica) is used for producing both meat and egg in many countries and as a model for animal research purposes. Two lines of Japanese quail that were differentiated by high body weight (HBW) and low body weight (LBW) had been improved by selection for body weight at four weeks of age. The objective of this study was to detect single nucleotide polymorphism (SNP) in Growth hormone (GH) and Insulin like Growth Factor -1 (IGF-1) genes in the two Japanese quails selected lines which based on the weight of the body at four weeks of age. DNA has been extracted from fifty blood samples by commercial kits and amplified by polymerase chain reaction (PCR). DNA sequencing revealed nucleotide polymorphisms between the two Japanese quails selected lines. The results of this investigation revealed that, one nucleotide change (T/C) in the intron 2 of GH gene. However, there were no nucleotide differences in IGF-1gene between the two selected lines. It concluded that, the SNP discovered in the GH gene may provide appropriate markers for associating researches of candidate genes with imperative economic measurements in Japanese quail. However, further studies are necessitating detecting mutation in another region of IGF-1.

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