Departmental Bulletin Paper Lethrinas nebulosus fish as a biomarker for petroleum hydrocarbons pollution in Red Sea : Alterations in antioxidants mRNA expression

Afifi, Mohamed  ,  Ali, Haytham A.  ,  Saber, Taghred M.  ,  El-Murr, Abd elhakeem

64 ( 2 )  , pp.S123 - S129 , 2016-04 , Graduate School of Veterinary Medicine, Hokkaido University
Total Petroleum Hydrocarbons (TPHs) are environmental contaminants that are released into the marine water via oil spills and industrial activities. The mRNA expression profile of some antioxidant genes in livers, gills, skin and muscles of Lethrinas nebulosus was used as biomarker of TPHs pollution in six areas at Jeddah and Yanbu coasts in Kingdom of Saudi Arabia (KSA). TPHs were determined in Red Sea water and sediments collected from the studied areas. Ten fish of similar sizes were collected from each area for the mRNA expression of super oxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPx) and glutathione-S-transferase (GST) and malondialdehyde (MDA) levels. The highest concentration of TPHs was observed in front of petromine refinery in Jeddah. The expression levels of antioxidant enzyme genes were highly significantly increased in the polluted areas. MDA levels were high in TPHs polluted areas. This study concluded that the expression profile of antioxidant enzyme genes in examined tissues of L. nebulosus could be utilized as a strong bioindicator of TPHs pollution in Jeddah and Yanbu coastal areas in KSA.

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