Journal Article Bone augmentation using a highly porous PLGA/β-TCP scaffold containing fibroblast growth factor-2

Yoshida, Takashi  ,  Miyaji, Hirofumi  ,  Otani, Kaori  ,  Inoue, Kana  ,  Nakane, Kazuyasu  ,  Nishimura, Hiroyuki  ,  Ibara, Asako  ,  Shimada, Ayumu  ,  Ogawa, Kosuke  ,  Nishida, Erika  ,  Sugaya, Tsutomu  ,  Sun, Ling  ,  Fugetsu, Bunshi  ,  Kawanami, Masamitsu

50 ( 2 )  , pp.265 - 273 , 2015-05 , Wiley-Blackwell
Background and objective: β-tricalcium phosphate (β-TCP), a bio-absorbable ceramic, facilitates bone conductivity. We constructed a highly porous three dimensional scaffold using β-TCP for bone tissue engineering and coated it with co-poly lactic acid/glycolic acid (PLGA) to improve the mechanical strength and biological performance. The aim of this study was to examine the effect of the implantation of the PLGA/β-TCP scaffold loaded with fibroblast growth factor-2 (FGF2) on bone augmentation. Material and methods: The β-TCP scaffold was fabricated by the replica method using polyurethane foam, then coated with PLGA. The PLGA/β-TCP scaffold was characterized by SEM, TEM, XRD, compressive testing, cell culture, and a subcutaneous implant test. Subsequently, a bone forming test was performed using fifty two rats. The β-TCP scaffold, PLGA-coated scaffold, and β-TCP scaffold and PLGA-coated scaffolds loaded with FGF2, were implanted into rat cranial bone. Histological observations were made at 10 and 35 days post-surgery. Results: SEM and TEM observations showed a thin PLGA layer on the β-TCP particles after coating. High porosity of the scaffold was exhibited after PLGA coating (> 90%), and the compressive strength of the PLGA/β-TCP scaffold was 6-fold greater than the non-coated scaffold. Good biocompatibility of the PLGA/β-TCP scaffold was found in the culture and implant tests. Histological samples obtained following implantation of PLGA/β-TCP scaffold loaded with FGF2 showed significant bone augmentation. Conclusion: The PLGA coating improved the mechanical strength of β-TCP scaffolds while maintaining high porosity and tissue compatibility. PLGA/β-TCP scaffolds in combination with FGF2 are bioeffective for bone augmentation.

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