||Bone augmentation using a highly porous PLGA/β-TCP scaffold containing fibroblast growth factor-2
Yoshida, Takashi Miyaji, Hirofumi ,
Otani, Kaori ,
Inoue, Kana ,
Nakane, Kazuyasu ,
Nishimura, Hiroyuki ,
Ibara, Asako ,
Shimada, Ayumu ,
Ogawa, Kosuke ,
Nishida, Erika Sugaya, Tsutomu ,
Sun, Ling Fugetsu, Bunshi ,
Journal of Periodontal Research
273 , 2015-05 , Wiley-Blackwell
Background and objective: β-tricalcium phosphate (β-TCP), a bio-absorbable ceramic, facilitates bone conductivity. We constructed a highly porous three dimensional scaffold using β-TCP for bone tissue engineering and coated it with co-poly lactic acid/glycolic acid (PLGA) to improve the mechanical strength and biological performance. The aim of this study was to examine the effect of the implantation of the PLGA/β-TCP scaffold loaded with fibroblast growth factor-2 (FGF2) on bone augmentation.
Material and methods: The β-TCP scaffold was fabricated by the replica method using polyurethane foam, then coated with PLGA. The PLGA/β-TCP scaffold was characterized by SEM, TEM, XRD, compressive testing, cell culture, and a subcutaneous implant test. Subsequently, a bone forming test was performed using fifty two rats. The β-TCP scaffold, PLGA-coated scaffold, and β-TCP scaffold and PLGA-coated scaffolds loaded with FGF2, were implanted into rat cranial bone. Histological observations were made at 10 and 35 days post-surgery.
Results: SEM and TEM observations showed a thin PLGA layer on the β-TCP particles after coating. High porosity of the scaffold was exhibited after PLGA coating (> 90%), and the compressive strength of the PLGA/β-TCP scaffold was 6-fold greater than the non-coated scaffold. Good biocompatibility of the PLGA/β-TCP scaffold was found in the culture and implant tests. Histological samples obtained following implantation of PLGA/β-TCP scaffold loaded with FGF2 showed significant bone augmentation.
Conclusion: The PLGA coating improved the mechanical strength of β-TCP scaffolds while maintaining high porosity and tissue compatibility. PLGA/β-TCP scaffolds in combination with FGF2 are bioeffective for bone augmentation.