||Biological Response to Nanostructure of Carbon Nanotube/titanium Composite Surfaces
Erika, Nishida Hirofumi, Miyaji ,
Junko, Umeda ,
Katsuyoshi, Kondoh ,
Hiroko, Takita ,
Izumi, Kanayama Saori, Tanaka ,
Akihito, Kato ,
Bunshi, Fugetsu Tsukasa, Akasaka ,
20 , 2015-06-30 , ナノバイオメディカル学会 , Nano Biomedical Society
Titanium (Ti) is frequently used as a biomaterial in dental and orthopedic implants and in bone
fixation devices. Effective modification of the Ti surface plays a crucial role in improving biocompatibility.
Carbon nanotubes (CNTs) are among the most interesting nanomaterials due to their
unique properties. In this study, we fabricated CNT-Ti composite surfaces by annealing Ti plates
covered by different sized CNTs (Nanocyl NC 7000, 9.5 nm diameter and VGCF-H, 150 nm diameter).
The properties of these surfaces were examined by scanning electron microscopy, X-ray
diffraction, energy dispersive X-ray spectroscopy, raman spectroscopy, contact angle measurement
and osteoblast-like cell seeding. In addition, samples were implanted into the subcutaneous tissue
of rats. The three-dimensional nanostructures of CNTs and creation of titanium carbide were evident
on the Ti surfaces, suggesting that the CNTs were well-anchored onto the Ti plates. CNT
modification promoted desirable cell behavior, including cell spreading and proliferation, especially
on the Nanocyl-modified surface. Inflammatory response was rarely observed on the Nanocyl
surface, but macrophage-like giant cells were frequently observed on the VGCF-H surface.
Therefore, the nanomorphology of narrow diameter CNTs provides a CNT-Ti composite surface
with good biocompatibility.