学術雑誌論文 Augmin shapes the anaphase spindle for efficient cytokinetic furrow ingression and abscission

Uehara, Ryota  ,  Kamasaki, Tomoko  ,  Hiruma, Shota  ,  Poser, Ina  ,  Yoda, Kinya  ,  Yajima, Junichiro  ,  Gerlich, Daniel W.  ,  Goshima, Gohta

27 ( 5 )  , pp.812 - 827 , 2016-03-01 , American Society for Cell Biology
ISSN:1059-1524
内容記述
During anaphase, distinct populations of microtubules (MTs) form by either centrosome-dependent or augmin-dependent nucleation. It remains largely unknown whether these different MT populations contribute distinct functions to cytokinesis. Here we show that augmin-dependent MTs are required for the progression of both furrow ingression and abscission. Augmin depletion reduced the accumulation of anillin, a contractile ring regulator at the cell equator, yet centrosomal MTs were sufficient to mediate RhoA activation at the furrow. This defect in contractile ring organization, combined with incomplete spindle pole separation during anaphase, led to impaired furrow ingression. During the late stages of cytokinesis, astral MTs formed bundles in the intercellular bridge, but these failed to assemble a focused midbody structure and did not establish tight linkage to the plasma membrane, resulting in furrow regression. Thus augmin-dependent acentrosomal MTs and centrosomal MTs contribute to nonredundant targeting mechanisms of different cytokinesis factors, which are required for the formation of a functional contractile ring and midbody.
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http://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/61445/1/Mol.%20Biol.%20Cell-2016-Uehara-812-27.pdf

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