学術雑誌論文 Characterization of a Cyanobacterial Chloride-pumping Rhodopsin and Its Conversion into a Proton Pump

Hasemi, Takatoshi  ,  Kikukawa, Takashi  ,  Kamo, Naoki  ,  Demura, Makoto

291 ( 1 )  , pp.355 - 362 , 2016-01-01 , American Society for Biochemistry and Molecular Biology (ASBMB)
ISSN:0021-92581083-351X
内容記述
Light-driven ion-pumping rhodopsins are widely distributed in microorganisms and are now classified into the categories of outward H+ and Na+ pumps and an inward Cl- pump. These different types share a common protein architecture and utilize the photoisomerization of the same chromophore, retinal, to evoke photoreactions. Despite these similarities, successful pump-to-pump conversion had been confined to only the H+ pump bacteriorhodopsin, which was converted to a Cl- pump in 1995 by a single amino acid replacement. In this study we report the first success of the reverse conversion from a Cl- pump to a H+ pump. A novel microbial rhodopsin (MrHR) from the cyanobacterium Mastigocladopsis repens functions as a Cl- pump and belongs to a cluster that is far distant from the known Cl- pumps. With a single amino acid replacement, MrHR is converted to a H+ pump in which dissociable residues function almost completely in the H+ relay reactions. MrHR most likely evolved from a H+ pump, but it has not yet been highly optimized into a mature Cl- pump.
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http://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/60922/1/MrHR_unmarked%20HUSCAP.pdf

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