Journal Article Multi-point Scanning Two-photon Excitation Microscopy by Utilizing a High-peak-power 1042-nm Laser

Otomo, Kohei  ,  Hibi, Terumasa  ,  Murata, Takashi  ,  Watanabe, Hirotaka  ,  Kawakami, Ryosuke  ,  Nakayama, Hiroshi  ,  Hasebe, Mitsuyasu  ,  Nemoto, Tomomi

31 ( 4 )  , pp.307 - 313 , 2015-04-10 , Japan Society for Analytical Chemistry
The temporal resolution of a two-photon excitation laser scanning microscopy (TPLSM) system is limited by the excitation laser beam's scanning speed. To improve the temporal resolution, the TPLSM system is equipped with a spinning-disk confocal scanning unit. However, the insufficient energy of a conventional Ti:sapphire laser source restricts the field of view (FOV) for TPLSM images to a narrow region. Therefore, we introduced a high-peak-power Yb-based laser in order to enlarge the FOV. This system provided three-dimensional imaging of a sufficiently deep and wide region of fixed mouse brain slices, clear four-dimensional imaging of actin dynamics in live mammalian cells and microtubule dynamics during mitosis and cytokinesis in live plant cells.

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