Liquid-Phase Detection of Biomaterial Using Magnetic Markers
高藤, 佳嗣 ,
入江, 康太 ,
吉田, 敬Enpuku, Keiji
7 , 2018-01 , 九州大学大学院システム情報科学研究院
We have been developing a liquid-phase immunoassay using magnetic markers and a supercon-ducting quantum interference device (SQUID). In this method, bound and free markers are magnetically differentiated by using the Brownian relaxation of the free markers. Hence, the time consuming washing process that is needed in the conventional optical method for Bound/Free (B/F) separation can be elimi-nated. Using the method, we demonstrated a detection of C-reactive protein (CRP). We obtained good correlation between the detected signal flux and the concentration of CRP. The minimum detectable con-centration was 1 pg/mL. Since the present sensitivity is limited by the blank signal caused by the aggre-gation of free markers, much improvement will be possible if this problem is solved.