Conference Paper Genetic diversity analysis and core collection formation for barley genetic resources in Egypt

Elakhdar, Ammar  ,  Kumamaru, Toshihiro  ,  Mansour, Mohamed  ,  Brueggeman, Robert  ,  Ludovic, Capo

The fundamental importance for breeding the elite barley materials is to identify the genetic diversity in that core collection to become more benefit for users as well as be validity in an association study. This study is seeking to measures the genetic diversity and population structure in 153 elite barley germplasm, represented the diversity in a total 472 core collection established through two decades ago. The genetic diversity of the core set was calculated to know the scope of diversity captured from the total collection. A total of 261 alleles were generated from 64 polymorphic loci with an average of 4.08 alleles per locus. The polymorphic information content (PIC) was wildly range (0.02 to 0.80) with a mean of 0.49 per locus. As well this, the average of the total expected heterozygosity (Ht) was 0.67 and ranged from 0.02 to 0.96. The 153 accessions were identified into three sub-populations; the genetic variations among them were 49%, while 51% for intra-population. Furthermore, Statistic FST among accessions (Intra-population) has indicated a significantly different (FST = 0.491, P>00.1). The higher polymorphic variation (76.81%) was observed in subgroups II, indicating more polymorphism existed than in cultivated and promising lines. The structure finds of the whole accession is in harmony with clustering approach using the SSR genotypic data of neighbor-joining tree and principal components analysis, which identified three sub-groups. / Therefore, the results demonstrate the genetic diversity of the accessions can be applied to predict approaches such as association analysis, classical mapping population development; parental line selection in breeding programs and hybrid development for exploiting the natural genetic variation exists in this population.

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