Journal Article Mapping quantitative trait loci for root development under hypoxia conditions in soybean (Glycine max L. Merr.)

Nguyen, Loc Van  ,  Takahashi, Ryoji  ,  Githiri, Stephen Mwangi  ,  Rodoriguez, Tito O.  ,  Tsutsumi, Nobuko  ,  Kajihara, Sayuri  ,  Sayama, Takasi  ,  Ishimoto, Masao  ,  Suematsu, Keisuke  ,  Abiko, Tomomi

2017-01 , Springer
Waterlogging is a major environmental stresslimiting soybean yield in wet parts of the world. Rootdevelopment is an important indicator of hypoxia tolerancein soybean. However, little is known about the geneticcontrol of root development under hypoxia. This studywas conducted to identify quantitative trait loci (QTLs)responsible for root development under hypoxia. Recombinantinbred lines (RILs) developed from a cross betweena hypoxia-sensitive cultivar, Tachinagaha, and a tolerantlandrace, Iyodaizu, were used. Seedlings were subjectedto hypoxia, and root development was evaluated with the value change in root traits between after and before treatments.We found 230 polymorphic markers spanning2519.2  cM distributed on all 20 chromosomes (Chrs.).Using these, we found 11 QTLs for root length (RL), rootlength development (RLD), root surface area (RSA), rootsurface area development (RSAD), root diameter (RD), andchange in average root diameter (CARD) on Chrs. 11, 12,13 and 14, and 7 QTLs for hypoxia tolerance of these roottraits. These included QTLs for RLD and RSAD betweenmarkers Satt052 and Satt302 on Chr. 12, which are importantmarkers of hypoxia tolerance in soybean; those QTLswere stable between 2  years. To validate the QTLs, wedeveloped a near-isogenic line with the QTL region derivedfrom Iyodaizu. The line performed well under both hypoxiaand waterlogging, suggesting that the region contains oneor more genes with large effects on root development.These findings may be useful for fine mapping and positionalcloning of gene responsible for root developmentunder hypoxia.

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